Summary
The nucleus is one of the most dynamic organelles in eukaryotes, and for yeast it has been estimated that about one third of all protein will reside in the nucleus at some point in time. The nuclear envelope with nuclear pore complexes embedded provides the barrier safeguarding the nuclear interior and chromatin. Traffic of a wide variety of macromolecules, like transcriptional regulators, ribosomal proteins and subunits, histones and mRNAs across the nuclear envelope is mediated by the nuclear pore complexes. The complex and dynamics nature of the nucleus makes it one of the more challenging organelles to study with proteomics methods. A comprehensive description of the proteins residing in the nucleus or at the nuclear envelope at specific conditions or cell cycle stage is key to answering fundamental research questions regarding nuclear functions, such as transcription regulation, nuclear positioning, and cell cycle control. We will study the nuclear envelope of budding yeast as a model system and specifically address which membrane proteins reside at the inner nuclear membrane and how the nuclear pore complex is involved in their targeting.