Summary
We have developed a platform for the simultaneous and high-throughput analysis of the expression potential of (membrane) proteins in multiple hosts. To increase the capacity of this platform even more, we aim at identifying and ultimately overcoming the bottlenecks limiting the functional overexpression of polytopic membrane proteins. We will use and develop proteomic methods to determine the consequences of membrane protein expression stress and to probe the changes in cells selected for increased protein production. The information will be used to engineer new generations of expression hosts, specifically designed for production of integral membrane proteins or protein secretion.