Summary
A current trend in high-throughput proteomics is to perform peptide-level studies on full lysates.  Often, protein fractionation is omitted and the entire sample is digested to create a pool containing hundreds of thousands of peptides which is directly accessible to mass spectrometry for peptide and then consequently protein identifications. The complexity of the analyte demands selective, fast and sensitive instrumentation alongside constant method development in sample preparation, fractionation, pre-concentration, chromatographic separation and detection. In the last few years a constant growth in proteome coverage has been achieved but maintaining a dynamic growth will require not only further optimization of current technologies but will also require developing separation/enrichment strategies which incorporate and exploit the physicochemical nature of peptides such as those that are phosphorylated or acetylated.  In this theme there will be a concerted effort to address two main areas; performance enhancement and introduction new separation/enrichment technologies.   Performance of current separations/enrichment technologies such as reversed phase, SCX and TiO2 will be enhanced.  While evaluation/ development of new strategies based on the some of the various HILIC forms will be performed.